Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Homo sapiens (Human) Active protein

CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.2
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  • Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Packages (Simulation)
  • Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Packages (Simulation)
  • APA045Hu61.jpg SDS-PAGE
  • Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Figure. Western Blot; Sample: APA045Hu61; Antibody: PAA045Hu06
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday . <br/>Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.The ED50 is 0.74ng/ml.

Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic SystemPubMed: 20391130
  • Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trialPubmed:24525316
  • Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
  • Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti …
  • Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal OriginPubmed: 31183654
  • The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­33285209
  • Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡­33612342

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