Active Complement Factor B (CFB) Rhesus monkey (Simian) Active protein

CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point7.5
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  • Active Complement Factor B (CFB) Packages (Simulation)
  • Active Complement Factor B (CFB) Packages (Simulation)
  • APC011Si02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Complement factor B (CFB), a 95-kDa protein, is a crucial catalytic element of the alternative pathway (AP) of complement. Upon cleavage by Complement Factor D (CFD), CFB splits into fragments Ba (inactive) and Bb, the latter forming the active C3 convertase (C3bBb). This enzyme amplifies complement activation by cleaving C3 into C3a/C3b, driving inflammation, opsonization, and membrane attack complex formation. CFB deficiency disrupts immune defense, linking it to infections and autoimmune disorders like atypical hemolytic uremic syndrome (aHUS).Thus a functional ELISA assay was conducted to detect the interaction of recombinant simina CFB and recombinant mouse CFD. Briefly, CFB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CFD-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CFB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant simina CFB and recombinant mouse CFD was shown in Figure 1, the EC50 for this effect is 0.015 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysisBioMed: 14712369
  • Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MSACS: pr300330z
  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Adipose tissue complement factor B promotes adipocyte maturation.pubmed:29137982
  • Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
  • Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han populationPubmed: 31857673
  • Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis33367879
  • Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse33410734
  • Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role34766501
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889

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