Active Connective Tissue Growth Factor (CTGF) Rattus norvegicus (Rat) Active protein

CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.2
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  • Active Connective Tissue Growth Factor (CTGF) Packages (Simulation)
  • Active Connective Tissue Growth Factor (CTGF) Packages (Simulation)
  • Active Connective Tissue Growth Factor (CTGF) Figure. Gene Sequencing (Extract)
  • APA010Ra01.jpg Figure. SDS-PAGE
  • Active Connective Tissue Growth Factor (CTGF) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of CTGF with ACTb.
Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. Besides, Actin Beta (ACTb) has been identified as an interactor of CTGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CTGF and recombinant rat ACTb. Briefly, CTGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CTGF and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

Figure.The adhere effect of CTGF on 3T3 cells.
(A) 3T3 cells were seeded into the well containting CTGF 1µg/mL and incubated for 1h at
37 ℃;
(B) 3T3 cells were seeded into the well without CTGF and incubated for 1h at 37℃;
To measure the effect of CTGF on cell adhesion, a general procedure performance as follows: 100μL PBS containing recombinant CTGF were incubated overnight at 4℃ in 96-well ELISA plates. Wells were blocked with 200µL PBS containing 3% BSA and then incubated for 1h at 37 ℃with 100µL PBS containing approximately 5x104 3T3 cells. Adherent cells were then fixed for 15 min with 5% formaldehyde and non-adherent cells were removed by washing each well three times with PBS. The remaining cells were incubated with 0.5% cristal violet for 10mins then counted at high magnification (×400) randomly (five fields for each well).

Figure.The adhere effect of CTGF on 3T3 cells.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis modelPubMed: 19824055
  • Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell functionPubMed: PMC2726461
  • Activation of JNK Signaling Mediates Connective Tissue Growth Factor Expression and Scar Formation in Corneal Wound HealingPlosone: 0032128
  • Clinical significance of inflammatory and fibrogenic cytokines in diabetic nephropathyScienceDirect: S0009912012000987
  • Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development?PubMed: S1053249812010121
  • The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
  • Concentrations of connective tissue growth factor in patients with nonalcoholic fatty liver disease: Association with liver fibrosisPubMed: 22846210
  • Clinical significance of connective tissue growth factor in hepatitis B virus-induced hepatic fibrosisPubMed: PMC3351780
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Cytokine levels as biomarkers of radiation fibrosis in patients treated with breast radiotherapyRo-journal: Source
  • Knock Out of S1P3 Receptor Signaling Attenuates Inflammation and Fibrosis in Bleomycin-Induced Lung Injury Mice ModelPubmed:Pmc4157792
  • Connective tissue growth factor and cardiac diastolic dysfunction: human data from the Taiwan Diastolic Heart Failure Registry and molecular basis by cellular and animal modelsPubmed:24464932
  • Connective Tissue Growth Factor is a Target of Notch Signaling in Cells of the Osteoblastic LineagePubmed:24792956
  • Disruption of collagen homeostasis can reverse established age-related myocardial fibrosisPubmed:25701883
  • Beneficial effects of combined ursodeoxycholic acid and angiotensin-II type 1 receptor blocker on hepatic fibrogenesis in a rat model of nonalcoholic steatohepatitisPubMed: 26190501
  • Suppression of connective tissue growth factor mediates the renoprotective effect of Sitagliptin rather than Pioglitazone in type 2 diabetes mellitusPubmed:27049870
  • TGF-β and Physiological Root Resorption of Deciduous Teeth.pubmed:28035998
  • Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
  • Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
  • The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidencepubmed:27531356
  • Molecular mechanisms underlying fibrosis and elastin destruction in childhood interstitial lung diseasespubmed:27686729
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
  • 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
  • Sustained β-AR stimulation induces synthesis and secretion of growth factors in cardiac myocytes that affect on cardiac fibroblast activationPubmed:29107793
  • Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller CellsPubmed:30123889
  • TGFβ mediates collagen production in human CRSsNP nasal mucosa‐derived fibroblasts through Smad2/3‐dependent pathway and CTGF induction and secretionPubmed: 30426494
  • Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with …Pubmed: 30413105
  • Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin MPubmed: 31249305
  • Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
  • CTGF Attenuates Tendon-Derived Stem/Progenitor Cell AgingPubmed: 31827530
  • Small-Dose Sunitinib Modulates p53, Bcl-2, STAT3, and ERK1/2 Pathways and Protects against Adenine-Induced NephrotoxicityPubmed: 33212804
  • Lysophosphatidic acid as a regulator of endometrial connective tissue growth factor and prostaglandin secretion during estrous cycle and endometrosis in the …Pubmed: 32943074
  • MicroRNAs and ventricular remodeling in aortic stenosisPubmed: 32682570
  • Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
  • High carbohydrate high fat diet causes arterial hypertension and histological changes in the aortic wall in aged rats: The involvement of connective tissue growth …34455071
  • Hepatocyte-specific Smad4 deficiency alleviates liver fibrosis via the p38/p65 pathway

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