Active Amiloride Binding Protein 1 (ABP1) Homo sapiens (Human) Active protein

AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.6
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  • Active Amiloride Binding Protein 1 (ABP1) Packages (Simulation)
  • Active Amiloride Binding Protein 1 (ABP1) Packages (Simulation)
  • Active Amiloride Binding Protein 1 (ABP1) Figure. Gene Sequencing (Extract)
  • APA656Hu61.png Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of DAO with DDC.
Diamine oxidase (DAO), also known as histaminase, is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. It is also secreted by eosinophils. In case of a shortage of diamine oxidase in the human body, it may appear as an allergy or histamine intolerance. Besides, Dopa Decarboxylase (DDC) has been identified as an interactor of DAO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DAO and recombinant human DDC. Briefly, DAO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to DDC-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DAO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DAO and DDC was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigsCas.zju: Source
  • A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in ratsPubmed:25022208
  • Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation ModelPubMed: 26064429
  • Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis.Pubmed:26804005
  • Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
  • A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injurypubmed:28522195
  • Serum Diamine Oxidase in Pseudoallergy in the Pediatric Populationpubmed:28804811
  • Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
  • Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and IronPubmed:29861488
  • Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsiaPubmed:29679053
  • Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trialPubmed:29277158
  • The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancerPubmed:29384543
  • LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD
  • Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease
  • Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
  • Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated micePubmed: 31749050
  • Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
  • HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in ratsPubmed: 31961020
  • The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancerPubmed: 33039631
  • Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in micePubmed: 32821439
  • Effects of fucoidan on tumor prevention and gut flora
  • Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats34587363
  • Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways
  • Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model

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