Active Endothelial NOS (eNOS) Homo sapiens (Human) Active protein

NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3, Endothelial

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.4
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  • Active Endothelial NOS (eNOS) Packages (Simulation)
  • Active Endothelial NOS (eNOS) Packages (Simulation)
  • APA868Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Endothelial Nitric Oxide Synthase (eNOS), also known as Nitric Oxide Synthase 3 (NOS3) or Constitutive NOS (cNOS), is an enzyme that plays a crucial role in maintaining cardiovascular health. It belongs to the nitric oxide synthase (NOS) family of enzymes, which are responsible for catalyzing the production of nitric oxide (NO) from L-arginine. eNOS is primarily expressed in endothelial cells, which line the interior of blood vessels.NOSIP can directly interact with eNOS and induce conformational change or spatial position change of eNOS, thus inhibiting enzyme activity of eNOS.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human eNOS and recombinant human NOSIP. Briefly, eNOS was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NOSIP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-eNOS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat eNOS and recombinant human NOSIP was shown in Figure 1, the EC50 for this effect is 0.05ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cellsScienceDirect: S0378874109004541
  • A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric OxidePubMed: 23359814
  • Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesityKnmu: Source
  • SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular DiseasesPlos: Source
  • L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSIONTdmu:Source
  • Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic SyndromePubmed:24798146
  • Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
  • A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:PubMed: 25785999
  • Comparative angioprotective effects of magnesium compoundsPubMed: 25127069
  • Peptides from hydrolysate of lantern fish (Benthosema pterotum) proved neuroprotective in vitro and in vivoscience:S1756464616300639
  • Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессуarticle:n
  • Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.pubmed:28088561
  • ThE REmOdELINg Of LEfT ATRIUm ANd vAsOdILATION fAcTORs chANgEs IN IschEmIc hEART fAILURE7038
  • Endothelial nitric oxide synthase deficiency influences normal cell cycle progression andapoptosis in trabecular meshwork cells.pubmed:27366677
  • SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.pubmed:28122311
  • SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from deathDOI:10.1016/j.biopha.2017.01.087
  • THE INFLUENCE OF THE ADDITIONAL USAGE OF L-ARGININE ON THE FACTORS OF VASODILATION AND THE CLINICAL DATA, COMPLICATED WITH CARDIAC DECOMPENSATION WITH REDUCED AND PRESERVED FRACTURE OF THE LEFT VENTRICLE THAT PATIENTS WITH STABLE CORONARY HEART DISEASE HAVEISSN: 0976-3376
  • Blunted Nitric Oxide Regulation in Tibetans under High Altitude Hypoxia10.1093:nsr
  • Molecular basis of vascular damage caused by cigarette smoke exposure and a new approach to the treatment: Alpha-linolenic acidPubmed:29579706
  • Ergothioneine prevents endothelial dysfunction induced by mercury chloridePubmed:29805489
  • Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarctionPubmed:29664427
  • Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne
  • Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in RatsDoi: 10.5152/eurasianjmed.2018.18160
  • Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant WomenPubmed: 30513890
  • Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility
  • Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathwayPubmed: 30975181
  • ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ …
  • Динамика показателей эндотелиальной дисфункции до и после операции у больных варикозной болезнью нижних конечностей
  • The placental growth factor attenuates intimal hyperplasia in vein grafts by improving endothelial dysfunctionPubmed: 31836533
  • Circadian fluctuations of endothelial nitric oxide synthase activity in females with rheumatoid arthritis: a pilot studyPubmed: 32025851
  • Circadian rhythms of endothelial nitric oxide synthase and toll-like receptors 2 production in females with rheumatoid arthritis depending on NOS3 gene polymorphism …Pubmed: 32297585
  • ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE …Pubmed: 32242853
  • Dynamics of endothelial dysfunction indices before and after operation in patients with varicose disease of the lower extremities
  • Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility
  • Оксидативный стресс и эндотелиальная дисфункция в развитии экспериментального радиоиндуцированного повреждения легких и их коррекция …
  • Angiogenic factors measured in aspirated placental tissue between the 10+ 6 and 18+ 3 weeks of gestation34798517
  • Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model ratsPubmed:34986543
  • Serum inducible and endothelial nitric oxide synthase in coronary artery disease patients with Type 2 Diabetes mellitusPubmed:35647851
  • Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory …Pubmed:35620404

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