Active Fetuin B (FETUB) Bos taurus; Bovine (Cattle) Active protein

FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.5
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  • Active Fetuin B (FETUB) Packages (Simulation)
  • Active Fetuin B (FETUB) Packages (Simulation)
  • APB860Bo01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Fetuin B (FETUB) is a secreted cystatin-family glycoprotein regulating extracellular matrix turnover. It functionally interacts with Collagen Type I Alpha 2 (COL1A2). Their crosstalk tunes type I collagen synthesis and fibril assembly, participating in fibrotic progression and stromal microenvironment remodeling.To detect the activity of recombinant FETUB, a functional ELISA assay was performed to evaluate the interaction between recombinant bovine FETUB and recombinant COL1A2. Briefly, COL1A2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FETUB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL1A2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. Measured by its binding ability in a functional ELISA. When recombinant FETUB is lmmobilized at 2 µg/mL(100 uLwell), the concentration of COL1A2 that produces 50% optimal binding response is found to be approximately 1.308 µg/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Serum fetuin B level increased in subjects of nonalcoholic fatty liver disease: a case-control studypubmed:27628583
  • Increased serum levels of fetuin B in patients with coronary artery diseasepubmed:28822077
  • Plasma fetuin-B concentrations are associated with insulin resistance and first-phase glucose-stimulated insulin secretion in individuals with different degrees of …Pubmed:29514765
  • Serum level of fetuin B is associated with osteoporosis: a 4-year prospective study in ChinaPubmed:29603688
  • Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and micePubmed: 30972177
  • Increased serum fetuin-B concentration is associated with HOMA-β and indices of liver steatosis in women with polycystic ovary syndrome: a pilot studyPubmed: 31307012
  • Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans33407555
  • Citrate high volume on-line hemodiafiltration modulates serum Interleukin-6 and Klotho levels: the multicenter randomized controlled study ¡°Hephaestus¡±33559851
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637

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