Active Fibroblast Growth Factor 11 (FGF11)

Fibroblast Growth Factor 11 (FGF11) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this gene has not yet been determined. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF11, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FGF11 and recombinant human FGFR1. Briefly, FGF11 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF11 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of rmFGF11 and rhFGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.