Active Fibroblast Growth Factor 15 (FGF15) Rattus norvegicus (Rat) Active protein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.6
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  • Active Fibroblast Growth Factor 15 (FGF15) Packages (Simulation)
  • Active Fibroblast Growth Factor 15 (FGF15) Packages (Simulation)
  • APL154Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Fibroblast growth factor 15 is a protein in mouse encoded by the Fgf15 gene. It is a member of the fibroblast growth factor (FGF) family but, like FGF19, FGF21 and FGF23, has endocrine functions. FGF15 subsequently acts on a cell surface receptor complex in hepatocytes to repress bile acid synthesis and gluconeogenesis, and to stimulate glycogen and protein synthesis.Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF15, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FGF15 and recombinant rat FGFR4. Briefly, FGF15 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to FGFR4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FGF15 and FGFR4 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21PubMed: 25774553
  • Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammationpubmed:27580383
  • Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis.pubmed:28130274
  • The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance10.1186/s12902-017-0211-5
  • The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in micePubmed:29468415
  • FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver TumorigenesisPubmed: 30745141
  • Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight
  • Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axisPubmed: 31626262
  • Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activationPubmed: 33036625
  • Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis34736501
  • Dynamics of the gut-liver axis in rats with varying fibrosis severityPubmed:35637968

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