Active Fibroblast Growth Factor 2, Basic (FGF2) Homo sapiens (Human) Active protein

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point9.7
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  • Active Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • Active Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • APA551Hu01.jpg Figure. SDS-PAGE
  • Active Fibroblast Growth Factor 2, Basic (FGF2) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Basic fibroblast growth factor (FGF2), also known as bFGF, is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Fibroblast growth factor receptor 4 (FGFR4) is a tyrosine kinase receptor for FGF2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF2 and recombinant human FGFR4. Briefly, biotin-linked FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF2 and recombinant human FGFR4 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelPubMed: 20438940
  • Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesisPubMed: PMC3254262
  • Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesisTandfonline: 644918
  • Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb IschemiaPubmed: 22266669
  • Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic RatsPubmed: 22091745
  • Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in RatsScialert: Source
  • Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart.PubMed: 22827927
  • Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesisPubMed: PMC3771850
  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesisPubmed: 23952606
  • Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness woundsPubmed: 23934870
  • Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate NeurotoxicityPubmed:25426952
  • Effects of Hemostatic Agents on Fibroblast CellsLww:Source
  • bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healingRsc:Source
  • Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryosPubmed:25530495
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in EwesScience: Article
  • Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosaarticle:S1742706115301410
  • Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scarsPubmed:26774564
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPApubmed:27456338
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts898221
  • FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesispubmed:28841454
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblastspubmed:28052053
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice10.1166/jbt.2017.1652
  • Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular RestorationPubmed:29630447
  • Increased serum FGF2 levels in first-episode, drug-free patients with schizophreniaPubmed: 30172685
  • Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cellsPubmed: 30359541
  • Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and …
  • HOXA9 is a novel myopia risk genePubmed: 30674274
  • Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
  • Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factorPubmed: 31669319
  • β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe DiseasePubmed: 31905906
  • Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditionsPubmed: 32323787
  • Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells
  • New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density33842739
  • Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia33878473
  • Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing33812576
  • Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨¹zerine etkisi
  • Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosisPubmed:35070009
  • Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats

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