Active Fibroblast Growth Factor 2, Basic (FGF2) Sus scrofa; Porcine (Pig) Active protein

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point9.7
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  • Active Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • Active Fibroblast Growth Factor 2, Basic (FGF2) Packages (Simulation)
  • APA551Po01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-β is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant pig FGF2 and recombinant pig CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelPubMed: 20438940
  • Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesisPubMed: PMC3254262
  • Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesisTandfonline: 644918
  • Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb IschemiaPubmed: 22266669
  • Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic RatsPubmed: 22091745
  • Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in RatsScialert: Source
  • Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart.PubMed: 22827927
  • Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesisPubMed: PMC3771850
  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesisPubmed: 23952606
  • Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness woundsPubmed: 23934870
  • Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate NeurotoxicityPubmed:25426952
  • Effects of Hemostatic Agents on Fibroblast CellsLww:Source
  • bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healingRsc:Source
  • Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryosPubmed:25530495
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in EwesScience: Article
  • Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosaarticle:S1742706115301410
  • Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scarsPubmed:26774564
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPApubmed:27456338
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts898221
  • FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesispubmed:28841454
  • 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblastspubmed:28052053
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice10.1166/jbt.2017.1652
  • Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular RestorationPubmed:29630447
  • Increased serum FGF2 levels in first-episode, drug-free patients with schizophreniaPubmed: 30172685
  • Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cellsPubmed: 30359541
  • Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and …
  • HOXA9 is a novel myopia risk genePubmed: 30674274
  • Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration
  • A novel xeno-free culture system for human retinal pigment epithelium cellsPubmed: 31024807
  • Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
  • Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factorPubmed: 31669319
  • β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe DiseasePubmed: 31905906
  • Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditionsPubmed: 32323787
  • Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells
  • New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density33842739
  • Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia33878473
  • Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing33812576
  • Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨¹zerine etkisi
  • Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosisPubmed:35070009
  • Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats

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