Active Fibronectin (FN) Sus scrofa; Porcine (Pig) Active protein

FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point6.8
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  • Active Fibronectin (FN) Packages (Simulation)
  • Active Fibronectin (FN) Packages (Simulation)
  • APA037Po01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Fibronectin (FN), a large, modular glycoprotein encoded by the FN1 gene, is a key component of the extracellular matrix (ECM) and plasma. Synthesized primarily by fibroblasts, hepatocytes, and endothelial cells, it exists in two major forms: soluble plasma FN and insoluble cellular FN. FN mediates critical cellular processes via its integrin-binding RGD motif, including cell adhesion, migration, proliferation, and differentiation. It also participates in ECM assembly, wound healing, and angiogenesis by bridging cells to other ECM components like collagen, fibrin, and proteoglycans. Dysregulated FN expression or function is linked to pathological conditions such as fibrosis, cancer metastasis, and cardiovascular diseases, making it a vital target for biomedical research. FN binds THBS1, a secreted glycoprotein, to modulate ECM stability and regulate cellular adhesion signaling pathways.Briefly, THBS1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-THBS1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately.Measured by its binding ability in a functional ELISA. When Recombinant FN is immobilized at 2 µg/mL(100 uLwell), the concentration of THBS1 that produces 50% optimal binding response is found to be approximately 0.054 µg/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidasePubMed: 19019916
  • Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
  • Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells.Pubmed: 24013407
  • Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathyScienceDirect: S0378874114002414
  • Inhibition by Female Sex Hormones of Collagen Gel Contraction Mediated by Retinal Pigment Epithelial CellsPubmed: 24609629
  • Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
  • Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytesPubmed:25333747
  • Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
  • In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative studyPubmed:25634132
  • In vivoPubMed: 25634132
  • Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate onNF-κB/leptin-insulin Axisin Obese Subjects with Non-alcoholic Fatty Liver DiseaseAjmbr: 3
  • Comparative proteomics of milk fat globule membrane in goat colostrum and mature milkPubmed:27173528
  • Maternal endothelial damage as a disorder shared by early preeclampsia, late preeclampsia and intrauterine growth restriction.pubmed:27865093
  • Effect of panax notoginseng saponins on efficacy and hemorrhagic transformation of rt-PA intravenous thrombolysis in patients with acute ischemic strokeArticle_en:CJFDTotal-XDJB201611014
  • Serum fbronectin distinguishes the early stages of hepatocellular carcinomapubmed:28842594
  • Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression10.3892/mmr.2017.7563
  • Tenomodulin is essential for prevention of adipocyte accumulation and fibrovascular scar formation during early tendon healing.pubmed:29022912
  • Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbitsPubmed:29932250
  • Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activitiesPubmed: 30530231
  • Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
  • TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K SignalingPubmed: 31111299
  • Three kinds of corneal host cells contribute differently to corneal neovascularization
  • Effects of autophagy on macrophage adhesion and migration in diabetic nephropathyPubmed: 31352855
  • Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
  • Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve RepairPubmed: 31758411
  • Molecular hydrogen regulates PTEN‐AKT‐mTOR signaling via ROS to alleviate peritoneal dialysis‐related peritoneal fibrosisPubmed: 31930571
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women?
  • Advanced glycation end-products associate with podocytopathy in type II diabetic patients
  • Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor functionPubmed:35586775

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