Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) Homo sapiens (Human) Active protein

FRCP2; Irisin; Fibronectin type III repeat-containing protein 2

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point6.2
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  • Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) Packages (Simulation)
  • Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) Packages (Simulation)
  • APN576Hu01.jpg Figure. SDS-PAGE
  • Active Fibronectin Type III Domain Containing Protein 5 (FNDC5) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. FNDC5 is a 12 kDa glycosylated polypeptide hormone that regulate glycolipid metabolism, cardiovascular homeostasis metabolism, stem cell differentiation, and neuronal development. Myostatin (MSTN) has been identified as an interactor of FNDC5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FNDC5 and recombinant human MSTN. Briefly, FNDC5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MSTN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FNDC5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FNDC5 and recombinant human MSTN was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.

Usage

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Serum irisin levels in new-onset type 2 diabetesPubMed: 23369227
  • Lower circulating irisin is associated with type 2 diabetes mellitusPubmed: 23619195
  • Inconsistency in circulating irisin levels: what is really happeningPubmed:24459033
  • Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in micePubmed:25199621
  • Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese micePubmed:25576856
  • Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysisPubMed: 25494632
  • Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancyPubMed: 26689349
  • Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-AnalysisPubmed:27300472
  • Physiology and role of irisin in glucose homeostasis.pubmed:28211512
  • Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese micepubmed:27638193
  • Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma pubmed:28867187
  • Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male micePubmed:29351550
  • The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer.Pubmed:29522296
  • Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative …Doi: 10.1016/j.redox.2018.10.019
  • Low serum irisin concentration is associated with poor outcomes in patients with acute pancreatitis and irisin administration protects against experimental acute …Pubmed: 31250660
  • Maternal serum, placental, and umbilical venous blood irisin levels in intrahepatic cholestasis of pregnancyPubmed: 31590596
  • Identification of irisin as a therapeutic agent that inhibits oxidative stress and fibrosis in a murine model of chronic pancreatitisPubmed: 32199226
  • Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver InjuryPubmed: 31976032
  • Serum Irisin Predicts Posthepatectomy Complications in Patients with Hepatocellular CarcinomaPubmed: 31976024
  • The myokine Irisin: localization and effects in swine late medium and large antral ovarian folliclePubmed: 33120167
  • The Combination of Aerobic and Resistance Exercise Induces Weight Loss via the PGC-1α/Irisin/UCP-1 Pathway
  • Evaluation of a recombinant bacillus calmette-guérin vaccine expressing P39-L7/L12 of Brucella melitensis: An immunization strategy against brucellosis in BALB/c …
  • Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients33755963
  • Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo …34822430
  • Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice34370270
  • Eight-week exercise program improved the quality of life of Alzheimer's patients through functional, cognitive, and biochemical parametersPubmed:35604540
  • Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent …Pubmed:35653888
  • Interaction of nitric oxide synthase and mitochondrial ATP-sensitive potassium channels in protective impacts of combination therapy with irisin …

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