Active Galanin Like Peptide (GALP)

Galanin-like peptide (GALP) is a 60 amino acid neuropeptide with a nonamidated C-terminus.GALP is primarily synthesized in the hypothalamic arcuate nucleus and the pituicytes of the posterior pituitary gland. The central effect of GALP is to regulate appetite and energy balance ,and it also participates in the regulation of the hypothalamic-pituitary-gonadal (HPG) axis.Besides,Insulin Degrading Enzyme (IDE) has been identified as an interactor of GALP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GALP and recombinant human IDE . Briefly, biotin-linked GALP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IDE-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GALP and IDE was shown in Figure 1, the EC50 for this effect is0.31ug/mL.