Active Gelsolin (GSN) Mus musculus (Mouse) Active protein

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.0
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  • Active Gelsolin (GSN) Packages (Simulation)
  • Active Gelsolin (GSN) Packages (Simulation)
  • APA372Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Gelsolin (GSN) is a ubiquitous, multifunctional actin-binding protein that plays a pivotal role in remodeling the cytoskeleton by severing, capping, and nucleating actin filaments. This calcium-dependent regulation is essential for critical cellular processes including cell motility, shape changes, and the clearance of cellular debris post-injury. By controlling the dynamics of actin assembly and disassembly, GSN influences a wide range of physiological functions from wound healing to platelet activation. Importantly, GSN directly binds to ACTC1, the primary actin isoform in cardiac muscle, to regulate the contractile apparatus and maintain heart function.To detect the activity of recombinant GSN , a functional ELISA assay was performed to evaluate the interaction between recombinant mouse GSN and recombinant human ACTC1.Briefly, GSN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse GSN and recombinant human ACTC1 was shown in Figure 1, the EC50 for this effect is 0.38µg/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of EpilepsySpringerLink: 3585r8h46q7r0171
  • Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injuryBioMed: 17422094
  • Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancerIEEE: 6098563
  • Investigation of the actin scavenging system in pre-eclampsiaPubmed: 24239294
  • Proteomic Study Reveals Plasma Protein Changes in Congenital Heart DiseasesPubmed: 24565402
  • The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot studyPubmed:Pmc4132929
  • Gelsolin: Role of a Functional Protein in Mitigating Radiation InjuryPubmed:25164111
  • Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer diseasePubMed: 25864780
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
  • Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.pubmed:28365030
  • Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental MethodsPubmed:30040720
  • Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young childrenPubmed:29427164
  • Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various …Pubmed:35327525

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