Active Gelsolin (GSN) Mus musculus (Mouse) Active protein

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point5.8
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  • Active Gelsolin (GSN) Packages (Simulation)
  • Active Gelsolin (GSN) Packages (Simulation)
  • APA372Mu03.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Gelsolin (GSN) is a highly conserved actin-binding protein widely expressed across eukaryotes, functioning as a key regulator of the actin cytoskeleton. It modulates actin filament assembly and disassembly through calcium-dependent severing, capping, and nucleation activities, thereby governing cellular processes like motility, shape change, cytokinesis, and vesicular trafficking. GSN exists in two isoforms: cytoplasmic GSN (regulating intracellular actin dynamics) and plasma GSN (a secreted form involved in extracellular actin scavenging and inflammation modulation). Dysregulation of GSN is associated with multiple pathologies, including neurodegenerative diseases (e.g., Alzheimer’s), cancer metastasis, cardiovascular disorders, and inflammatory conditions. Its role in maintaining cytoskeletal homeostasis and extracellular matrix balance makes it a significant molecule in both physiological and pathological contexts.Besides,CTTN has been identified as an interactor of GSN, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GSN and recombinant human CTTN. Briefly, biotin-linked GSN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to CTTN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GSN and CTTN was shown in Figure 1, the EC50 for this effect is 0.30181µg/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of EpilepsySpringerLink: 3585r8h46q7r0171
  • Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injuryBioMed: 17422094
  • Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancerIEEE: 6098563
  • Investigation of the actin scavenging system in pre-eclampsiaPubmed: 24239294
  • Proteomic Study Reveals Plasma Protein Changes in Congenital Heart DiseasesPubmed: 24565402
  • The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot studyPubmed:Pmc4132929
  • Gelsolin: Role of a Functional Protein in Mitigating Radiation InjuryPubmed:25164111
  • Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer diseasePubMed: 25864780
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
  • Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.pubmed:28365030
  • Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental MethodsPubmed:30040720
  • Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young childrenPubmed:29427164
  • Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various …Pubmed:35327525

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