Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- UOM
- FOB US$ 236.00 US$ 590.00 US$ 1,180.00 US$ 3,540.00 US$ 8,850.00
- Quantity
Overview
Properties
- Product No.APB090Ha01
- Organism SpeciesHamster (Chinese hamster) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayTumor immunityHepatology
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point7.9
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Activity test

GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant chinese hamster GSTP1, mix gentliy. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chinese hamster GSTp1 is >6500 pmol/min/µg.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Citations
- Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular CarcinomaPubMed: PMC3619117
- Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With NeurocysticercosisPubmed:27021019
- 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineeringart00007
- Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative statePubmed:30019218
- Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
- The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands