Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- UOM
- FOB US$ 230.00 US$ 576.00 US$ 1,152.00 US$ 3,456.00 US$ 8,640.00
- Quantity
Overview
Properties
- Product No.APB090Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayTumor immunityHepatology
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point7.1
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Activity test

Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1

Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Citations
- Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular CarcinomaPubMed: PMC3619117
- Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With NeurocysticercosisPubmed:27021019
- 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineeringart00007
- Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative statePubmed:30019218
- Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats
- The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands