Active Glycine Dehydrogenase (GLDC)

Glycine Dehydrogenase (GLDC) is a key mitochondrial enzyme involved in the catabolism of glycine. It catalyzes the oxidative decarboxylation of glycine, playing an important role in one-carbon metabolism. GLDC provides one-carbon units for various cellular processes like nucleotide synthesis and methylation reactions.Besides,Apolipoprotein H (APOH) has been identified as an interactor of GLDC,Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse GLDC and recombinant mouse AGXT. Briefly, GLDC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AGXT-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GLDC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse GLDC and recombinant mouse AGXT was shown in Figure 1, the EC50 for this effect is 0.097ug/mL.