Active Golgi Phosphoprotein 2 (GOLPH2) Mus musculus (Mouse) Active protein

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.2
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  • Active Golgi Phosphoprotein 2 (GOLPH2) Packages (Simulation)
  • Active Golgi Phosphoprotein 2 (GOLPH2) Packages (Simulation)
  • APB668Mu01.jpg SDS-PAGE
  • Active Golgi Phosphoprotein 2 (GOLPH2) Figure. Western Blot; Sample: Recombinant GP73, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissuesMcponline: 023507
  • Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar CarcinomaAjied: Ajied_System_Files
  • Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients.handle:123456789
  • Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinomapubmed:29113278
  • The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian studyPubmed: 32198440
  • Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformationPubmed: 32171652
  • Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡­33540642
  • Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C

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