Active Gremlin 1 (GREM1) Homo sapiens (Human) Active protein

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point9.0
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  • Active Gremlin 1 (GREM1) Packages (Simulation)
  • Active Gremlin 1 (GREM1) Packages (Simulation)
  • APC128Hu01.png Figure. SDS-PAGE
  • Active Gremlin 1 (GREM1) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Gremlin 1 (GREM1) is an inhibitor in the TGF-beta signaling pathway. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GREM1 and recombinant human BMP2. Briefly, GREM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to BMP2-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-GREM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human GREM1 and recombinant human BMP2 was shown in Figure 1，the EC50 for this effect is 0.1~0.562 ug/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial HypertensionPubMed: 25926293
  • Overexpression of Gremlin promotes non-small cell lung cancer progressionPubMed: 26392110
  • Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patientsPubMed: 26539964
  • Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patientsPubmed:26539964
  • BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAHPubmed:27187737
  • GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and InvasivenessPubmed:26834210
  • The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.pubmed:28266688
  • Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.pubmed:28327551
  • Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial …rcmb.2015-0402OC
  • Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cellspubmed:28260028
  • Platelets as a novel source of Gremlin-1: Implications for thrombo inflammationpubmed:27929199
  • Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic GlomerulonephritisPubmed: 31053735
  • Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage RegenerationPubmed: 31878307
  • Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathwayPubmed: 32186934
  • Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesaPubmed:35441352
  • CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenibPubmed:35638711

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