Active Chemokine (C-X-C Motif) Ligand 2 (CXCL2) Rattus norvegicus (Rat) Active protein

GROb; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Growth Regulated Oncogene Beta

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.7
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  • Active Chemokine (C-X-C Motif) Ligand 2 (CXCL2) Packages (Simulation)
  • Active Chemokine (C-X-C Motif) Ligand 2 (CXCL2) Packages (Simulation)
  • APB603Ra01.jpg SDS-PAGE
  • Active Chemokine (C-X-C Motif) Ligand 2 (CXCL2) Figure. Western Blot; Sample: Recombinant GROb, Rat.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced chemokine release in both TRAIL-resistant and TRAIL-sensitive cells via nuclear factor kappa BPubMed: 19120450
  • HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa KeratitisPlos: Source
  • Identification of human exercise-induced myokines using secretome analysisPubmed:24520153
  • Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose TissuePubMed: 26324179
  • Bubble CPAP support after discontinuation of mechanical ventilation protects rat lungs with ventilator-induced lung injuryPubmed:26800273
  • The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37RaPubmed:26912204
  • Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activationpubmed:28873369
  • Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activityPubmed:29953010
  • Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in MicePubmed: 31077413
  • IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancerPubmed: 31173199
  • The Clinicopathological Significance of the CXCR2 Ligands, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8 in Gastric CancerPubmed: 31810929
  • Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitisPubmed: 32709888
  • Assessment of acute toxicological effects of molybdenum (IV) disulfide nano-and microparticles after single intratracheal administration in ratsPubmed: 32629262
  • Vitamin D3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidificationPubmed:34989311

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