Active Heme Oxygenase 1 (HO1) Bos taurus; Bovine (Cattle) Active protein

HMOX1; Hsp32; HMOX1D

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7.2
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  • Active Heme Oxygenase 1 (HO1) Packages (Simulation)
  • Active Heme Oxygenase 1 (HO1) Packages (Simulation)
  • APA584Bo61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Heme Oxygenase 1 (HO1) is an enzyme that catalyzes the rate-limiting step in heme degradation, converting heme into biliverdin, carbon monoxide (CO), and free iron. Expressed primarily in the spleen and liver, HO1 is induced by oxidative stress, inflammation, and hypoxia. It plays critical roles in cellular protection by mitigating oxidative damage, modulating inflammation, and promoting iron recycling. HO1's products, particularly CO and biliverdin (later reduced to bilirubin), exhibit antioxidant, anti-apoptotic, and vasodilatory properties. Its activity is tightly regulated and linked to pathophysiological processes, including ischemia-reperfusion injury, atherosclerosis, and neurodegenerative diseases.Biliverdin Reductase B (BLVRB) collaborates with HO1 , which rapidly reduces biliverdin to bilirubin. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant bovine HO1 and recombinant human BLVRB . Briefly, biotin-linked HO1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to BLVRB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant bovine HO1 and recombinant human BLVRB was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport SyndromePubMed: 25777063
  • Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing SpondylitisPubmed:27314037
  • Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
  • Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidityPubmed:26824276
  • Sulforaphane protects against sodium valproate–induced acute liver injurypubmed:28112972
  • Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
  • S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
  • Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
  • Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.pubmed:28068636
  • Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis.pubmed:28050757
  • Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
  • Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patientspubmed:28655573
  • Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolismpubmed:28694840
  • Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinomapubmed:29101811
  • Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese childrenDOI: 10.1111/apa.14106
  • Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1pubmed:28589248
  • Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathwaypubmed:28670881
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …10.1016:j.talanta.2018.01.044
  • The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder10.1016:j.pharep.2018.06.001
  • Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
  • Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicityPubmed:29753615
  • Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced …10.1063:1.5033415
  • Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stressDoi: 10.22038/IJBMS.2018.31716.7714
  • Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in ratsPubmed: 30530041
  • Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazolePubmed: 30790573
  • Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis
  • Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase …
  • Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cells
  • Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosisPubmed: 31465731
  • Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosisPubmed: 31600583
  • The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathwayPubmed: 32526285
  • Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentPubmed: 28593326
  • Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury33793806
  • In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanismPubmed:35594934
  • Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECHPubmed:35697292

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