Active Histone Deacetylase 1 (HDAC1) Mus musculus (Mouse) Active protein

HDAC1; GON10; HD1; RPD3; RPD3L1

Add to Cart Distributors
Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.2
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Active Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • Active Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • APC206Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Histone deacetylase 1 (HDAC1) is an enzyme which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Besides, Specificity Protein 1 (Sp1) has been identified as an interactor of HDAC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HDAC1 and recombinant human Sp1. Briefly, HDAC1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to Sp1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HDAC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450 nm immediately. The binding activity of of recombinant mouse HDAC1 and recombinant human Sp1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylasePubmed:25609369
  • Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cellsScience: Article
  • Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal ratsPubMed: 26410212
  • Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's diseasePubMed: 25931268
  • Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cellsS1016319016300295

Recommend products