Active Immunoglobulin E (IgE) Homo sapiens (Human) Active protein

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.4
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  • Active Immunoglobulin E (IgE) Packages (Simulation)
  • Active Immunoglobulin E (IgE) Packages (Simulation)
  • APA545Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Immunoglobulin E (IgE) is one of the members the 5 classes of immunoglobulins (IgM, IgG, IgD, IgA, IgE).It is produced by plasma cells and accounts for less than 0.01% of the total immunoglobulin.IgE has a unique chemical structure and various physiological functions, such as type I hypersensitivity reactions, parasitic infections, autoimmune processes, and venom protection. Besides,Cluster of Differentiation 79B (CD79B) has been identified as an interactor of IgE, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IgE and recombinant human CD79B.Briefly, IgE was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD79B-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IgE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human IgE and recombinant human CD79B was shown in Figure 1, the EC50 for this effect is 0.075ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infectionPubMed: 23280722
  • IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infectionPubMed: 23354321
  • Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammationPubmed: 24313384
  • Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic EczemaPubMed: 25511806
  • Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammationPubMed: 26123423
  • The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway.pubmed:27484042
  • Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
  • Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lungpubmed:27992791
  • Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
  • Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt.pubmed:28586019
  • Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice10.1016:j.jaut.2018.07.003
  • Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse modelPubmed:29655095
  • Investigation of hypersensitivity potential of diacetyl by determining cytokine profilesPubmed:29233007
  • Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
  • Effects of catalpol on bronchial asthma and its relationship with cytokinesPubmed: 30536454
  • Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat ModelPubmed: 30781605
  • Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
  • Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis
  • Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expressionPubmed: 31737193
  • Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injectionPubmed: 32302806
  • Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
  • Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in VivoPubmed: 32335278
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
  • Biomarkers of Allergic Asthma and their Association with Serum Parameters
  • Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice33706133
  • Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure34090073
  • Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
  • Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal PolypsPubmed:35075349
  • Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthmaPubmed:35549595

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