Active Insulin Like Growth Factor 1 (IGF1) Homo sapiens (Human) Active protein

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.8
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  • Active Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • Active Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • Active Insulin Like Growth Factor 1 (IGF1) Gene sequencing
  • APA050Hu61.jpg SDS-PAGE
  • Active Insulin Like Growth Factor 1 (IGF1) Figure. SDS-PAGE; Sample: Active recombinant IGF1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IGF-1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IGF1 was shown in Figure2. It was obvious that IGF1 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 8.66~17.19 ng/mL.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapyPubMed: 20119855
  • Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male ratsPubMed: 20204528
  • The vagus nerve as a regulator of growth hormone secretionPubMed: 21056594
  • Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in RatsPubMed: 21651821
  • Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress.PubMed: 20224062
  • The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelPubMed: 20438940
  • Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidismPubMed: 21546289
  • Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch periodWiley: source
  • Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent WayWiley: source
  • Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in RatsMdpi: 8562
  • Protective Effect of Amlodipine on Rat Bone Tissue after OrchidectomyKarger: 335491
  • Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal PigletsDegruyter: Source
  • BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogsPubMed: PMC3232391
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian folliclesScielo: Source
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitroScienceDirect: S0093691X13001659
  • Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybridsScienceDirect: S0956566313005629
  • The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsInformahealthcare: Source
  • A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor BiomarkersTandfonline: Source
  • CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASILEmbrapa: Source
  • Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal MuscleCepebr:Source
  • The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.Pubmed:24629477
  • Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary SyndromePubmed:25667201
  • Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • The use of herb mix and lucerne to increase growth rates of dairy heifersSystem: Files
  • Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in PigsJournals: 1000183
  • The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat ModelPubMed: 25708898
  • Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVESWp-Content: Uploads
  • CaracterizaTeses: Disponiveis
  • Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane ReceptorsPubmed:27128575
  • Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn ratsPubmed:27291304
  • A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampusPubmed:27008926
  • Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in MicePubmed:27003941
  • Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen.pubmed:27605972
  • Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training.pubmed:27880816
  • Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors.pubmed:28216608
  • Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway.pubmed:28177762
  • Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsyarticle:10.1007
  • GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化10697
  • Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloespubmed:28785907
  • Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1pubmed:28627605
  • Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy pubmed:28078614
  • Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
  • Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
  • Studies on quality attributes of skimmed colostrum powderP-ISSN: 2349–8528
  • Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats10.1186/s13287-017-0739-3
  • A metabolite of leucine (β-hydroxy-β-methylbutyrate) given to sows during pregnancy alters bone development of their newborn offspring by hormonal modulation10.1371/journal.pone.0179693
  • A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injurypubmed:28522195
  • Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Micepubmed:28853013
  • Effect of dietary protein intake on the body composition and metabolic parameters of neutered dogspubmed:29152244
  • Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair.pubmed:28540539
  • Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.pubmed:28959186
  • Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF-1 Signaling Pathway.pubmed:28708285
  • An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signalingPubmed:29627530
  • Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transferPubmed:29482569
  • Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
  • Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition …10.1016:j.rvsc.2018.04.009
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
  • 26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumorsPubmed:29755191
  • Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs.:
  • Circulating concentrations of insulin like growth factor-1 in female dogs with spontaneous mammary tumours10.21521:mw.6054
  • Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagyPubmed:29423092
  • Effect of porcine somatotropin on metabolism, testicular size and sperm characteristics in young boars10.1590:1678-4162-9481
  • Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigsPubmed: 30203098
  • Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin systemPubmed: 30659706
  • The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKBPubmed: 30784932
  • Association between Insulin-Like Growth Factor-I Levels and the Disease Progression and Anemia in Visceral LeishmaniasisPubmed: 30761980
  • Light-dark rhythms during incubation of broiler chicken embryos and their effects on embryonic and post hatch leg bone developmentPubmed: 30682088
  • Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling PathwayPubmed: 30987295
  • Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bullsPubmed: 30914211
  • Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryoPubmed: 31059537
  • Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
  • Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes
  • Effect of betaine supplementation on growth performance, nutrient intake and expression of IGF-1 in Karan Fries heifers during thermal stressPubmed: 31711708
  • Effects of a diet enriched with eicosapentaenoic, docosahexaenoic and glutamine on cytokines as immunological markers for systemic inflammation in bitches before …Pubmed: 31637790
  • The association between serum insulin-like growth factor 1 and cognitive impairments in patients with schizophreniaPubmed: 31839419
  • BST Stimulation Induces Atrophy and Changes in Aerobic Energy Metabolism in Rat Skeletal Muscles—The Biphasic Action of Endogenous GlucocorticoidsPubmed: 32316389
  • Fenugreek Stimulates the Expression of Genes Involved in Milk Synthesis and Milk Flow through Modulation of Insulin/GH/IGF-1 Axis and Oxytocin SecretionPubmed: 33081164
  • Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon HealingPubmed: 32370144
  • Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?Pubmed: 32331362
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and
  • INFLUENCE OF OMEGA–3 RICH FATTY ACID ENRICHED FEED ON BIOCHEMICAL MARKERS AND POST PARTUM REPRODUCTIVE PERFORMANCE OF …
  • Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care33469783
  • Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water33428732
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 40433562568
  • New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density33842739
  • The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­33381190
  • The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets33435211
  • Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡­
  • The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study34348809
  • Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes34359233

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