Active Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- UOM
- FOB US$ 320.00 US$ 800.00 US$ 1,600.00 US$ 4,800.00 US$ 12,000.00
- Quantity
Overview
Properties
- Product No.APA071Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryCytokineApoptosis
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point6.5
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Activity test

IL1α (Interleukin-1 alpha) is a member of the interleukin 1 cytokine family. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces cell apoptosis. It is reported that exposure of MCF-7 cells to certain concentration of IL1α results in inhibition of cell growth. Thus, an cell proliferation assay of MCF-7 was conducted with the addition of IL1α. MCF-7 cells were seeded overnight at a density of 5,000 cells/well, and then treated with or without various concentrations of IL1α for 72h, then cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37°C.<br/>Inhibition of MCF-7 cell proliferation after incubation with IL1α for 72h observed by inverted microscope was shown in Figure 1.

Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of IL1α for 72h. The mean OD value of MCF-7 assessed by CCK-8 was shown in Figure 2. It was obvious that IL1α significantly decreased cell viability of MCF-7 cells.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Spike RBD Protein (S-RBD)
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Protein A
Citations
- Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathyPubmed: 24662024
- Expression of S100 family proteins in neonatal rats with sepsis and its significancePubMed: 25973048
- Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
- Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection.pubmed:27502394
- Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathypubmed:28333148
- Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection propertiesDOI: 10.1111/jocd.12349
- Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheetspubmed:28811665
- Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis.pubmed:29191221
- Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitusPubmed:30066828
- Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammationPubmed:29243061
- Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclagePubmed:29357727
- Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation SystemsPubmed: 30970054
- The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency
- Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
- Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein …Pubmed: 31560476
- The toxicity of alumina nanoparticles to immune system in micePubmed: 32162999
- Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regenerationPubmed: 32308770
- IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathwaysPubmed: 33107399
- ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathwayPubmed: 32908567
- Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattlePubmed: 32583225
- Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis34256094
- Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells34780748
- Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation34944269
- Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation …Pubmed:34944269