Active Interleukin 1 Family, Member 9 (IL1F9) Rattus norvegicus (Rat) Active protein

IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2

Add to Cart Distributors
Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7.9
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Active Interleukin 1 Family, Member 9 (IL1F9) Packages (Simulation)
  • Active Interleukin 1 Family, Member 9 (IL1F9) Packages (Simulation)
  • APL621Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1F9 can induced IL-8 production in HacaT cells. To test the bioactivity of IL-1F9, HacaT cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of IL-1F9 for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of HacaT cells increased significantly after stimulated with IL-1F9 have shown in Figure1.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic MyofibroblastsPubMed: 26562662
  • Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndromePubmed:29314201
  • For Review Only
  • Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζPubmed: 31863918
  • Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver DiseasePubmed:35212223
  • Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitisPubmed:35481896

Recommend products