Active Interleukin 1 Receptor Antagonist (IL1RA) Mus musculus (Mouse) Active protein

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point8.5
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  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Gene sequencing
  • APA223Mu01.jpg SDS-PAGE
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Figure. Western Blot; Sample: Recombinant IL1RA, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

IL1RA (interleukin-1 receptor antagonist) is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Besides, mouse IL1R1 and rat IL1R1 share similarities in amino acid sequence with the identity of 85.6%. Thus, a binding ELISA assay was constructed to detect the association of recombinant mouse IL1RA with recombinant rat IL1R1. Briefly, IL1RA were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1R1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL1RA with IL1R1 was shown in Figure 1 and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel diseaseScienceDirect: S1090023312000962
  • Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horsesResearchgate:Source
  • Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1Science: Article
  • A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol ConcentrationsScience: Article
  • Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horsereceive:101658
  • Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrainpubmed:29141788
  • A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
  • Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
  • Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel diseasePubmed:29572935
  • M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in MicePubmed: 33041798

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