Active Interleukin 1 Receptor Antagonist (IL1RA) Rattus norvegicus (Rat) Active protein

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.7
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  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • Active Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • APA223Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

The interleukin-1 receptor antagonist (IL-1RA) is a member of the interleukin 1 cytokine family. IL1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. IL-1RN is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Thus, a binding ELISA assay was conducted to detect the interaction of recombinant rat IL-1RN and recombinant rat IL-1R1. Briefly, IL-1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL-1R1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL-1RN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1R1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel diseaseScienceDirect: S1090023312000962
  • Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horsesResearchgate:Source
  • Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1Science: Article
  • A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol ConcentrationsScience: Article
  • Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horsereceive:101658
  • Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrainpubmed:29141788
  • A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
  • Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
  • Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel diseasePubmed:29572935
  • M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in MicePubmed: 33041798

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