Active Interleukin 12B (IL12B) Homo sapiens (Human) Active protein

p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.5
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  • Active Interleukin 12B (IL12B) Packages (Simulation)
  • Active Interleukin 12B (IL12B) Packages (Simulation)
  • Active Interleukin 12B (IL12B) Figure . Gene Sequencing (extract)
  • APA058Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin 12B (IL12B)encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities.Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Interleukin 12B can combind with Interleukin 12 Receptor Beta 1 (IL12Rb1). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL12B and recombinant human IL12Rb1. Briefly, biotin-linked recombinant human IL12B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to IL12Rb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL12B and IL12Rb1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ.Pubmed: 23564769
  • Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress.pubmed:27384769
  • Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritisS1756464616303528
  • Photothermal-triggered Controlled Drug Release From Mesoporous Silica nanoparticles Based On Base-pairing Rules
  • Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal PigsPubmed: 31963653
  • Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553

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