Active Interleukin 17B (IL17B)

IL-20; Cytokine Zcyto7; Neuronal interleukin-17-related factor

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 198.00 US$ 495.00 US$ 990.00 US$ 2,970.00 US$ 7,425.00
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Overview

Properties

Product No.APB700Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsCell culture; Activity Assays.
Research use only
DownloadInstruction Manual
CategoryCytokine Infection immunity

Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits Freeze-dried powder, Purity > 95%
Isoelectric Point9.7

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Packages (Simulation)
Packages (Simulation)

SDS-PAGE
Figure. Western Blot; Sample: Recombinant IL17B, Human.

ISO9001: 2008, ISO13485: 2003 Registered

Activity test

IL17B (Interleukin-17B) is is a T cell-derived cytokine that shares sequence similarity with IL17, which has been proven to mediate potent inflammatory immune response. Besides, increasing evidence suggests that IL17 may play a critical role in various kinds of liver diseases, including hepatocellualar carcinoma. Thus a stimulation assay has been conducted to detect the bioactivity of IL17B. HepG2 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (250ng/mL, 500ng/mL) of IL17B for 20h and IL-8 levels in the cell supernatant were determined by ELISA.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.