Active Interleukin 17F (IL17F)

Interleukin 17F (IL17F) is a member of Interleukin 17 (IL-17) family proteins which is secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. IL17F is a disulfide-linked dimer. It is pro-inflammatory cytokine that induce local cytokine production and is involved in the regulation of immune functions.To Measure the binding ability of recombinant human IL17F,a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL17F and recombinant mouse Interleukin 17 Receptor E (IL17RE). Briefly, biotin-linked IL17F were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL17RE-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human IL17F and recombinant mouse IL17RE was shown in Figure 1, the EC50 for this effect is 0.80ug/mL.