Active Interleukin 3 Receptor Alpha (IL3Ra) Mus musculus (Mouse) Active protein

CD123; IL3R-A; IL3RY; IL3RAY; IL3R; IL3RAY; IL3RY; hIL-3Ra

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.1
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  • Active Interleukin 3 Receptor Alpha (IL3Ra) Packages (Simulation)
  • Active Interleukin 3 Receptor Alpha (IL3Ra) Packages (Simulation)
  • APB505Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin 3 receptor alpha (IL3Ra), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity mouse IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. Both the alpha and the beta subunits are members of the cytokine receptor superfamily. A binding ELISA assay was conducted to detect the interaction of recombinant mouse Interleukin 3 Receptor Alpha and recombinant mouse Interleukin 3. Briefly, IL3Ra were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL3Ra pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL3Ra and IL3 was shown in Figure 1, the EC50 was 10.66 ug/ml.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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