Active Interleukin 33 (IL33) Homo sapiens (Human) Active protein

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.5
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  • Active Interleukin 33 (IL33) Packages (Simulation)
  • Active Interleukin 33 (IL33) Packages (Simulation)
  • Active Interleukin 33 (IL33) Figure. Gene Sequencing (Extract)
  • APB980Hu01.png Figure. SDS-PAGE
  • Active Interleukin 33 (IL33) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of IL33 with IL1RL1.
Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarctionPubMed: PMC3604606
  • CXCL13 blockade attenuates lupus nephritis of MRL/lpr micePubMed: 26456520
  • Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
  • The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.pubmed:27348082
  • IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligopubmed:27388717
  • Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
  • Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)10.1080:13102818.2018.1471361
  • Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer172341
  • Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposurePubmed: 30413212
  • Interleukin-33 serum levels in postmenopausal women with osteoporosisPubmed: 30846811
  • Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2sPubmed: 32101749
  • Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmaticsPubmed: 32141256
  • Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus34395633
  • Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005
  • Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling

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