Active Interleukin 33 (IL33) Mus musculus (Mouse) Active protein

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.1
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  • Active Interleukin 33 (IL33) Packages (Simulation)
  • Active Interleukin 33 (IL33) Packages (Simulation)
  • APB980Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin 33 (IL33) is a dual-function protein that acts both as a traditional cytokine and an intracellular nuclear factor. It is primarily released by endothelial and epithelial cells upon cellular damage or stress, serving as an "alarmin" to alert the immune system. IL33 signals through its receptor ST2 to drive type 2 immune responses, which are crucial in allergic inflammation, host defense against parasites, and tissue repair.Besides,Thymic Stromal Lymphopoietin (TSLP) has been identified as an interactor of IL33, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IL33 and recombinant human TSLP. Briefly, IL33 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TSLP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL33 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse IL33 and recombinant human TSLP was shown in Figure 1, the EC50 for this effect is 0.23 µg/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarctionPubMed: PMC3604606
  • CXCL13 blockade attenuates lupus nephritis of MRL/lpr micePubMed: 26456520
  • Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
  • The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.pubmed:27348082
  • IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligopubmed:27388717
  • Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
  • Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)10.1080:13102818.2018.1471361
  • Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer172341
  • Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposurePubmed: 30413212
  • Interleukin-33 serum levels in postmenopausal women with osteoporosisPubmed: 30846811
  • Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2sPubmed: 32101749
  • Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmaticsPubmed: 32141256
  • Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus34395633
  • Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005
  • Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling

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