Active Interleukin 5 (IL5) Homo sapiens (Human) Active protein

E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7.5
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  • Active Interleukin 5 (IL5) Packages (Simulation)
  • Active Interleukin 5 (IL5) Packages (Simulation)
  • APA078Hu62.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin-5 (IL-5), a secreted glycoprotein, is a member of  the hematopoietic family. Interleukin 5 has been shown to  stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 ℃. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 ℃ . Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of recombinant human IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhagePubMed: PMC3383056
  • Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsArticle_14936_6.Html:
  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthmaPubmed:27289030
  • Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Cavia (Guinea pig )inea PigsPubmed:27130219
  • Interleukin-31 expression and relation to disease severity in human asthmapmc:PMC4783779
  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthmapubmed:27289030
  • Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
  • Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
  • Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')Pubmed: 19363516
  • ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
  • Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in micePubmed: 30929267
  • An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
  • Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expressionPubmed: 31737193
  • Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
  • Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic micePubmed: 32521453
  • Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • MiR-142-5p directly targets cyclin-dependent kinase 5-mediated upregulation of the inflammatory process in acquired middle ear cholesteatoma34875451
  • Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows

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