Active Interleukin 6 Receptor (IL6R)

CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 200.00 US$ 500.00 US$ 1,000.00 US$ 3,000.00 US$ 7,500.00
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Overview

Properties

Product No.APB815Mu02
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsCell culture; Activity Assays.
Research use only
DownloadInstruction Manual
CategorySignal transduction CD & Adhesion molecule Infection immunity

Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits Freeze-dried powder, Purity > 97%
Isoelectric Point6.5

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Packages (Simulation)
Packages (Simulation)

Figure. SDS-PAGE; Sample: Active recombinant IL6R, Mouse.
Figure. Western Blot; Sample: Recombinant IL6R, Mouse.

ISO9001: 2008, ISO13485: 2003 Registered

Activity test

IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

Increment services

Citations

Interleukin‐6 and interleukin‐6 receptor expression, localization, and involvement in pain‐sensing neuron activation in a mouse intervertebral disc injury modelPubMed: 26010074
The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthmaPubmed:26997259
Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-¦Â1/Smad pathway33893003