Active Interleukin 9 (IL9) Homo sapiens (Human) Active protein

HP40; P40; TCGFIII; TCGF3; T Cell Growth Factor III; P40 T-Cell And Mast Cell Growth Factor; P40 Cytokine; Homolog Of Mouse T Cell And Mast Cell Growth Factor 40

Add to Cart Distributors
Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.1
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Active Interleukin 9 (IL9) Packages (Simulation)
  • Active Interleukin 9 (IL9) Packages (Simulation)
  • APA081Hu01.png Figure. SDS-PAGE
  • Active Interleukin 9 (IL9) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa protein which was originally identified in mice as a T cell growth factor and is a member of the common γ-chain-receptor cytokine family, with other members including IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is produced predominantly by helper T cells such as Th2 and Th9 cells. It normally functions through the activation of a JAK/STAT pathway and plays a critical role in immunity and the pathogenesis of cancer. S100 Calcium Binding Protein A6 (S100A6) is a high affinity receptor for IL-9. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL-9 and recombinant mouse S100A6. Briefly, IL-9 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A6-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human IL-9 and recombinant mouse S100A6 was shown in Figure 1, the EC50 for this effect is 0.09 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Involvement of T-Helper 9 Activation in a Mouse Model of Allergic RhinitisPubmed:29982264
  • Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related PancytopeniaPubmed: 32455141
  • miR-143/145 inhibits Th9 cell differentiation by targeting NFATc133446394

Recommend products