Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)

IDH; IDP; ICD-M; IDHM; mNADP-IDH; Isocitrate dehydrogenase [NADP], mitochondrial; Oxalosuccinate decarboxylase

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ US$ US$ US$ US$
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Overview

Properties

Product No.APH838Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsCell culture; Activity Assays.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits Freeze-dried powder, Purity > 90%
Isoelectric Point8.3

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Packages (Simulation)
Packages (Simulation)

Figure. SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Isocitrate Dehydrogenase 2 (IDH2) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP as the electron acceptor and the other using NAD . The protein encoded by this gene is the NADP dependent isocitrate dehydrogenase found in the mitochondrial. The activity of recombinant human IDH2 was measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The reaction was performed in 25 mM Tris, 0.5 mM MnCl2, 5 mM DTT, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDH2 (diluted by Assay Buffer) to 50 µL of substrate mixture consisted of 1 mM NADP and 2 mM isocitric acid. The final well serves as a negative control with no IDH2, replaced with 50μl assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human IDH2 is >200 pmol/min/µg.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.