Active Kallistatin (KAL)
SERPINA4; KLST; KST; PI4; Serpin Peptidase Inhibitor Clade A Member 4; Tissue Kallikrein Inhibitor; Kallikrein Binding Protein; Peptidase inhibitor 4
- UOM
- FOB US$ 220.00 US$ 550.00 US$ 1,100.00 US$ 3,300.00 US$ 8,250.00
- Quantity
Overview
Properties
- Product No.APD392Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsCell culture; Activity Assays.
Research use only - DownloadInstruction Manual
- CategoryInfection immunity
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 80%
- Isoelectric Point8.1
Sign into your account
Share a new citation as an author
Upload your experimental result
Review

Contact us
Please fill in the blank.
Activity test

Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to KAL-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
-
BCA Protein Quantification Kit
-
Molecular Mass Marker for Protein
-
Monoclonal Antibody Customized Service
-
Polyclonal Antibody Customized Service
-
Protein Activity Test Experiment Service
-
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
-
Buffer
-
Lentivirus Packaging Experiment Service
-
Adenovirus Packaging Experiment Service
-
Real Time PCR Experimental Service
-
Spike RBD Protein (S-RBD)
-
Protein G
-
Protein A