Active Laminin Gamma 1 (LAMC1)

Laminin Gamma 1 (LAMC1), also known as LAMB2, is a member of the laminin family of proteins. LAMC1 is expressed in a variety of tissues and organs, including liver, kidney, heart, and brain. Its expression level is usually regulated by a variety of factors, such as hormones, growth factors, and cytokines. At the cellular level, LAMC1 is mainly distributed in the basement membrane and extracellular matrix of cells, and plays the role of cell adhesion, migration, differentiation and signal transduction by binding to cell surface receptors. Besides, Laminin Alpha 2 (LAMa2) has been identified as an interactor of LAMC1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human LAMC1 and recombinant mouse LAMa2. Briefly, LAMC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LAMa2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LAMC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human LAMC1 and recombinant mouse LAMa2 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.