Active Matrix Metalloproteinase 2 (MMP2) Homo sapiens (Human) Active protein

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.2
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  • Active Matrix Metalloproteinase 2 (MMP2) Packages (Simulation)
  • Active Matrix Metalloproteinase 2 (MMP2) Packages (Simulation)
  • Active Matrix Metalloproteinase 2 (MMP2) Gene sequencing
  • APA100Hu02.jpg SDS-PAGE
  • Active Matrix Metalloproteinase 2 (MMP2) Figure. Western Blot; Sample: Recombinant MMP2, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control.
Result 1: Gelatin hydrolysis by recombinant human MMP2 was shown in figure 1.

Usage

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Bone morphogenetic protein-2: a potential regulator in scleral remodelingPubMed: PMC2605409
  • Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis VirusPubmed: source
  • The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary MenZjrms: Source
  • Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cellsRsmjournals: Source
  • Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental strokePubMed: 21679435
  • Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cellsPubmed: 23544611
  • The unwounded skin remodeling in animal models of diabetes types 1 and 2.Pubmed: 24020818
  • RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cellsPubmed: 24681113
  • Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients?Pubmed: 23994841
  • Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative coursePubmed: 23585206
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritisPubmed:25824718
  • Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasionPubmed:25078863
  • In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative studyPubmed:25634132
  • Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In VitroPubmed:25767513
  • A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary MenPhypha:Source
  • Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the HorseJournals: Mi
  • MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1Content: Asp
  • In vivoPubMed: 25634132
  • Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathwaysPubMed: 26191138
  • Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cellsPubMed: 25884474
  • Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+PubMed: 26433066
  • Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosisPubMed: 26412140
  • Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in ratsDoi: Abs
  • A comparative study to evaluate the effect of limited access dressing on diabetic ulcersArticle: 2347-9264
  • Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγPubMed: 26703570
  • CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthmaJournal:27699
  • 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathwayPubmed:26782341
  • Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migrationprofile:57162df608aebf0697f1843d
  • Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic RatsPubmed:26851971
  • Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infectionPubmed:26925446
  • MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1Pubmed:27160777
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
  • Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugatesscience:S0956566315304474
  • Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia.pubmed:28050662
  • Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.pubmed:28348451
  • Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damagepubmed:27761275
  • Pleiotropic effects of survivin in vascular endothelial cells.pubmed:27373518
  • Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction.pubmed:27938355
  • MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1.pubmed:27160777
  • MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Linepublication:306067745
  • Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings.pubmed:27853690
  • Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblastsart00008
  • Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activitypubmed:28091828
  • MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1pubmed:27743351
  • Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosispubmed:28821422
  • MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1pubmed:28573504
  • The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cellspubmed:29224019
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
  • Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9DOI:10.1111/fcp.12294
  • Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosispubmed:28665955
  • Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway10.1080/17435390.2017.1357213
  • CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma.pubmed:28507585
  • Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritispubmed:28901438
  • Syntenin-targeted peptide blocker inhibits progression of cancer cellsPubmed:29857221
  • Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodelingPubmed:29572844
  • Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia …10.1002:jcb.26849
  • Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral NeuropathyPubmed:29704151
  • sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasionPubmed:30022839
  • CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular RemodelingPubmed:29984229
  • Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamidePubmed:30058214
  • Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitroPubmed:29328476
  • miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells10.3892:ol.2018.9170
  • HMGB1-RAGE signaling pathway in pPROMPubmed:29673663
  • MMP-2 and TIMP-2 in patients with heart failure and chronic kidney diseasePubmed:29915813
  • Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF.Pubmed: 30464333
  • Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
  • MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1
  • Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
  • Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular …Pubmed: 31464166
  • MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16Pubmed: 31796150
  • Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosisPubmed: 31980722
  • Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial CellsPubmed: 32205213
  • Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertensionPubmed: 33175289
  • Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling …Pubmed: 32745509
  • Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantationPubmed: 32671254
  • A novel role of VEPH1 in regulating AoSMC phenotypic switchingPubmed: 32342520
  • Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats
  • Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah
  • Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep
  • MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic33488296
  • GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression33613720
  • Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia33381317
  • Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice34618622
  • Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human …34762897
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
  • Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer34650982

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