Active Matrix Metalloproteinase 7 (MMP7) Homo sapiens (Human) Active protein

MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.8
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  • Active Matrix Metalloproteinase 7 (MMP7) Packages (Simulation)
  • Active Matrix Metalloproteinase 7 (MMP7) Packages (Simulation)
  • APA102Hu61.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Matrix Metalloproteinase 7 (MMP7) is a member of the matrix metalloproteinases (MMPs) family which are zinc and calcium dependent endopeptidases. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. Thus we have chosen casein-zymography to measure the activity of MMP7. Briefly, various concentrations of MMP7 (10ug, 5ug, 1ug, 0.1ug ,0.01ug) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat-polyacrylamide gel (SDS-PAGE; 15% gels) containing casein (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP7 would hydrolyze casein nearby, which was indicated by the white bands on the gel. In this experiment, we use heat-denatured MMP7 protein as negative control, and trypsin (1ug/ml) as positive control. The result was shown in figure 1.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin modelPubMed: 20649573
  • Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis VirusPubmed: source
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infectionPubmed:26925446
  • Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosisPubmed:26993522
  • The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cellspubmed:29224019
  • Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult10371/137708
  • Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia.pubmed:29167395
  • Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary AtresiaPubmed: 30153340
  • Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary AtresiaPubmed: 30853207
  • Serum MMP-7 in the Diagnosis of Biliary AtresiaPubmed: 31604829
  • Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated ratsPubmed: 31710895
  • The synthetic toxin biliatresone causes biliary atresia in micePubmed: 32681026
  • Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­33924683
  • A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer34215253

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