Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Homo sapiens (Human) Active protein

ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.5
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  • Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Packages (Simulation)
  • Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Figure. Gene Sequencing (Extract)
  • APC300Hu01.png Figure. SDS-PAGE
  • Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

MANF (Mesencephalic astrocyte-derived neurotrophic factor) is a prosurvival protein that protects the cells when applied intracellularly in vitro or extracellularly in vivo. Recently, MANF has also been proven to inhibit cell proliferation and ER stress-induced cell death and also affects cell size and morphology under certain conditions. Thus the bioactivity of human recombinant MANF were detected using U87-MG. Briefly, U87-MG cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of MANF. After incubated for 48h, cells were observed by inverted microscope and were shown in Figure 1. It was obvious that MANF inhibited cell proliferation of U87-MG and affected cell size and morphology. (A)U7-MG cells cultured in DMEM, stimulated with 0.01ng/mL MANF for 48h;
(B)Unstimulated U87-MG cells cultured in DMEM for 48h.
Figure 1. Inhibition of U87-MG cell proliferation after stimulated with MANF.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.pubmed:28216543
  • Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
  • Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance10.1507:endocrj.EJ16-0472
  • MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage
  • Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway33856409
  • The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …34234505

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