Active Myocilin (MYOC)

Myocilin (MYOC) is a secreted protein found in human aqueous humor (AH). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component. Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway. To test the effect of MYOC on cell proliferation, AGS cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant mouse MYOC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse MYOC for 72h. The result was shown in Figure 1. It was obvious that MYOC significantly increased cell viability of AGS cells. The ED50 of recombinant mouse MYOC is 1.033μg/ml.