Active Myostatin (MSTN) Rattus norvegicus (Rat) Active protein

GDF8; Growth Differentiation Factor 8

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.8
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  • Active Myostatin (MSTN) Packages (Simulation)
  • Active Myostatin (MSTN) Packages (Simulation)
  • APB653Ra01.jpg Figure. SDS-PAGE
  • Active Myostatin (MSTN) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Figure. The binding activity of MSTN with FSTL3.
Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat MSTN and recombinant rat FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized RatsSapub:Source
  • Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in micePubMed: 26539241
  • Tumor inoculation site affects the development of cancer cachexia and muscle wastingPubMed: 26016447
  • Induction of transient tenogenic phenotype of high density cultured human dermal fibroblastsPubMed: 25748814
  • Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary DiseasePubmed:26998756
  • The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized RatsPubmed:27272029
  • Irisin and Myostatin Levels in Patients with Graves' Disease.pubmed:27986127
  • Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Ratspubmed:29242963
  • Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis.pubmed:28740214
  • Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigsPubmed: 30203098
  • Predictive value of serum myostatin for the severity and clinical outcome of heart failurePubmed: 31056368
  • Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar ratsPubmed: 31960436
  • Myostatin and muscle atrophy during chronic kidney diseasePubmed: 32974666
  • Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance …34868459

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