Active Neutrophil gelatinase-associated lipocalin (NGAL) Sus scrofa; Porcine (Pig) Active protein

Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.3
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  • Active Neutrophil gelatinase-associated lipocalin (NGAL) Packages (Simulation)
  • Active Neutrophil gelatinase-associated lipocalin (NGAL) Packages (Simulation)
  • APB388Po01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant pig NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant pig NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 µL of the different concentrations of Fe(DHBA)3 and 50 µL of 100 ug/ml rpNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant pig Lipocalin-2 can bind >1 µM of Fe(DHBA)3.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancyPubMed: PMC3794962
  • Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 PathwayPubmed: 24369464
  • Lipocalin-2: Response To Resistance Training in Obese Young MenIjssjournal: Source
  • Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young MenTums: Source
  • Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlapPubmed: 23794464
  • Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathyPubmed:24802166
  • Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental studyPubmed:25812822
  • 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义Wjgnet:Source
  • Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
  • Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese MenBiomedpharmajournal:Source
  • A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source
  • 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
  • Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancyPubmed:Pmc4260273
  • Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseasePubmed:Pmc4043430
  • Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:24916275
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Increased cardiac index attenuates septic acute kidney injury: a prospective observational studyPubMed: 25745357
  • 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义Journal: Fulltext
  • Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in RatsPubMed: 25909514
  • Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:26714833
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
  • Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
  • Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.pubmed:27775820
  • Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)pubmed:28659153
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
  • Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
  • Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
  • Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activityPubmed:30036488
  • Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic NephropathyPubmed:29642511
  • Improved approach for normothermic machine perfusion of cold stored kidney graftsPubmed:30018731
  • Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
  • Potential Novel Biomarkers of Obstructive Nephropathy in Children with HydronephrosisDoi: 10.1155/2018/1015726
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicinePubmed: 30871515
  • Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascadePubmed: 31197431
  • A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
  • Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
  • Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory ArrestPubmed: 31578351
  • The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …Pubmed: 32980930
  • Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transitionPubmed: 32633566
  • CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …
  • Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy
  • Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers33423326
  • Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice33398418
  • Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury33604384
  • Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer34104101
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
  • Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts34944524
  • SERS based Y-shaped aptasensor for early diagnosis of acute kidney injuryPubmed:35733690

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