Active Paraoxonase 1 (PON1) Homo sapiens (Human) Active protein

ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.1
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  • Active Paraoxonase 1 (PON1) Packages (Simulation)
  • Active Paraoxonase 1 (PON1) Packages (Simulation)
  • Active Paraoxonase 1 (PON1) Gene sequencing
  • APA243Hu01.jpg SDS-PAGE
  • Active Paraoxonase 1 (PON1) Figure. Western Blot; Sample: Recombinant PON1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic SyndromePubMed: 20205513
  • Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndromePubMed: 21704607
  • Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and AdolescentsWiley: source
  • High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudyPubMed: 21887280
  • Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudyPubMed: 21696738
  • Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteomePubMed: PMC3480889
  • Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activitiesPubmed: 23510814
  • Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitusPubmed: 23680251
  • Paraoxonase responses to exercise and niacin therapy in men with metabolic syndromePubmed:25180827
  • Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of ratsPubmed:25548375
  • A single session of aerobic exercise influences paraoxonase 1 activity and concentrationArticulo: 345738764042
  • Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with CirrhosisPubMed: 25789864
  • Association of lipid profile with serum PON1 concentration in patients with chronic kidney diseasePubmed:26888625
  • The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorderscience:article
  • Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertensionpubmed:27668323
  • Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian populationpubmed:29254890
  • A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellituspubmed:29156090
  • Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosisS0021967317311858
  • The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.pubmed:28623707
  • Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar RatsISSN: 2381-7224
  • Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levelspubmed:28808839
  • Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney DiseasePubmed:29368426
  • Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in RatsPubmed: 30251228
  • Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancerPubmed: 30607521
  • Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktailsPubmed: 30661467
  • Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancyPubmed: 31162828
  • Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
  • Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intakePubmed: 31757567
  • Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteinsPubmed: 31783242
  • The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation …Pubmed: 33148285
  • PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patientsPubmed: 32556934
  • The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction
  • Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans33407555
  • Vitamin D status and systemic redox biomarkers in adults with obesity30973911
  • Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men34917230

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