Active Parathyroid Hormone (PTH) Homo sapiens (Human) Active protein

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.1
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  • Active Parathyroid Hormone (PTH) Packages (Simulation)
  • Active Parathyroid Hormone (PTH) Packages (Simulation)
  • APA866Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Parathyroid hormone (PTH) is an 84-amino-acid peptide hormone that is secreted by the parathyroid gland. PTH plays an osteogenic role by acting directly on mesenchymal stem cells, cells with an osteoblastic lineage, osteocytes, and T cells. It also participates as an osteoclast by indirectly acting on osteoclast precursor cells and osteoclasts and directly acting on T cells. PTH is major endocrine regulators of vitamin D metabolism. It proves, with data, this hormone a potent down-regulator of VDR. A functional binding ELISA assay was conducted to detect the interaction of recombinant human PTH and recombinant mouse VDR. Briefly, PTH was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to VDR-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PTH and recombinant mouse VDR was shown in Figure 1, the EC50 for this effect is 0.26 ug/mL.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient DietPubMed: 19915804
  • Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed miceScienceDirect: S0899900709004808
  • Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized RatsKoreaMed: source
  • Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parametersPubmed: 22173025
  • The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
  • Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats.PubMed: 22439438
  • Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia?PubMed: PMC3679169
  • Effect of the “protein diet” and bone tissue.Pubmed: 24483972
  • Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivoAacrjournals: Source
  • Effect of the “protein diet” and bone tissueAulamedica: Source
  • Effect of whole body vibration on healthy rat plasma parametersEbscohost: Source
  • Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional ricketsPubmed:25051233
  • An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic CancerPubMed: 26266370
  • The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized RatsPubMed: 26639847
  • Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized RatArticle: Hksobf_2015_V46N2_167
  • Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
  • Cell-matrix signals specify bone endothelial cells during developmental osteogenesis.pubmed:28218908
  • Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting.pubmed:27442254
  • Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat10.22192/ijarbs.2017.04.04.018
  • Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient micePubmed:29363324
  • Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
  • CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblastsPubmed: 31679847
  • A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female RatsPubmed: 32852547
  • Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts.
  • PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES
  • Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats34373766
  • Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector34599290
  • Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney InjuryPubmed:35456008
  • Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease

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