Active Parathyroid Hormone Related Protein (PTHrP) Mus musculus (Mouse) Active protein

PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point10.1
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  • Active Parathyroid Hormone Related Protein (PTHrP) Packages (Simulation)
  • Active Parathyroid Hormone Related Protein (PTHrP) Packages (Simulation)
  • Active Parathyroid Hormone Related Protein (PTHrP) Gene sequencing
  • APA819Mu01.jpg SDS-PAGE
  • Active Parathyroid Hormone Related Protein (PTHrP) Figure. Western Blot; Sample: Recombinant PTHrP, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Characterization of a new renal cell carcinoma bone metastasis mouse modelPubMed: 20443133
  • Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated.PubMed: 22133004
  • PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast InhibitionPubMed: PMC3753447
  • Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclastsPubmed: 23673814
  • Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatmentPubmed:24463094
  • Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyPubmed:24943060
  • 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone LossPubMed: 25859665
  • A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysisPubMed: 26135836
  • Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation.Pubmed:27312487
  • Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectinpubmed:27740628
  • Lactation induces increases in the RANK/RANKL/OPG system in maxillary bonePubmed:29382610
  • A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic MicePubmed: 31600911
  • Identification of a potential gene target for osteoarthritis based on bioinformatics analysesPubmed: 32571421
  • Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related …
  • Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни
  • Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡­33510128
  • Effects of whole-body vibration training in a cachectic C26 mouse model34732809
  • The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signalsPubmed:35334154

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